Rat apolipoprotein AI (Apo-AI) ELISA kit

Cat. No.: OB0625WXX-116

Assay Type: Quantitative competitive ELISA

Target Species: Rat

Assay Target: Apo-AI

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Rat apolipoprotein AI (Apo-AI) ELISA kit

INQUIRY Datasheet MSDS

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Product Overview

Description: Rat apolipoprotein AI (Apo-AI) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of Apo-AI in rat with a specificity of 0.1 ng/mL.

Assay Principle: The assay samples and buffer are incubated with the Apo-AI-HRP conjugate in a precoated plate for one hour. At the end of the incubation period, the samples are decanted, and the wells are washed five times. The wells are then incubated with HRP enzyme substrate. The products of the enzyme-substrate reaction form a blue color complex. Finally, the reaction is stopped by the addition of a stop solution, which turns the solution yellow. The color intensity is measured spectrophotometrically at 450 nm on a microplate reader. The color intensity is inversely proportional to the concentration of Apo-AI because the Apo-AI in the sample competes with the Apo-AI-HRP conjugate for the binding site of the anti-Apo-AI antibody. Since the number of binding sites is limited, as more sites are occupied by Apo-AI in the sample, fewer sites remain to bind to the Apo-AI-HRP conjugate. A standard curve is plotted between color intensity (O.D.) and standard concentration. The concentration of Apo-AI in each sample is derived from this standard curve.

Background: Apo-AI is the major protein component of high-density lipoprotein (HDL) in plasma and is involved in the transport of cholesterol from tissues to the liver for excretion.

Synonyms: APOA1, Apolipoprotein A1, Apolipoprotein A-I, HPALP2

Applications: Rat apolipoprotein AI (Apo-AI) ELISA kit is an important tool for the quantitative detection of Apo-AI in various biological samples (serum, plasma, cell culture supernatants, body fluid, tissue homogenate) of rats.

Research Area: Metabolism; Obesity research

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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