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Alpha-amylase and beta-amylase activity assay kit
Cat. No.:
OB0125WXX-003
Assay Type:
Activity assay
Target Species:
Universal
Assay Target:
Alpha-Amylase and Beta-amylase
Size:
Add to basket
Product Overview
Description:
Alpha-amylase and Beta-amylase activity assay kit is an enzyme activity test kit that detects AchE, in the range of 1.225-493 U/mL
Assay Principle:
Under heating conditions, reducing sugars react with 3,5-dinitrosalicylic acid to form a brownish-red substance. Amylase activity can be calculated by measuring the OD value at 540 nm.
Background:
Amylase is a general term for hydrolases and glycogenases, which generally act on soluble starch, straight-chain starch, glycogen, etc., and are widely found in animals and others.
Synonyms:
Alpha-Amylase; α-amylase; 1,4-α-D-glucan glucanohydrolase; Salivary amylase (ptyalin); Beta-Amylase; β-amylase; 1,4-α-D-glucan maltohydrolase; EC 3.2.1.1； EC 3.2. 1.2
Applications:
Alpha-amylase and Beta-amylase activity assay kit is used to measure α-amylase activity in serum, saliva, plasma, and tissue samples.
Research Area:
Food safety; Enzyme; Obesity
Specification
Sample Type:
Serum; Plasma; Saliva; Tissue
Kit Components:
Substrate; Chromogenic agent; Standard (10 mg/mL); Microplate; Plate sealer
Detection Method:
Colorimetric method
Detection Range:
0.01-0.56 U/mL
Sensitivity:
0.008 U/mL
Precision (Intra-assay):
2.8% (Average)
Precision (Inter-assay):
3.6% (Average)
Reaction Time:
30 min
Recovery:
0.97
Storage:
Store at 2-8°C.