AdipoUpX™ Human SIM1 MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human SIM1 MMLV Particle (Overexpression) is a high-titer MMLV particle designed for the efficient and stable overexpression of the human SIM1 gene. As a transcription factor critical for hypothalamic development and satiety regulation, its stable expression is an ideal asset for investigating the genetic pathways of hyperphagic obesity. The viral system features a modular design, offering high flexibility for customizing functional components. Comprehensive quality control is performed on every batch to ensure product efficacy and the reproducibility of your experimental results.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: SIM1

Full Name: SIM bHLH transcription factor 1

Gene Symbol: bHLHe14

Gene ID: 6492

RefSeq ID-1: NP_001361698.1

RefSeq ID-2: NM_001374769.1

Summary: SIM1 is a human homolog of the Drosophila single-minded gene, which plays a key role during embryonic development. Expression analyses indicate that SIM1 transcripts are predominantly detected in fetal tissues, including the developing kidney. Given the critical involvement of the Drosophila sim gene in neurogenesis, SIM1 has been proposed to contribute to human brain development and craniofacial patterning. Disruption of SIM1 function has been implicated in neurodevelopmental abnormalities and disorders affecting appetite control and body weight regulation.

AdipoUpX™ Human SIM1 MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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