AdipoUpX™ Human RAI1 MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human RAI1 MMLV Particle (Overexpression) is a ready-to-use retroviral vector particle professionally engineered for the stable overexpression of the human RAI1 gene. Haploinsufficiency of RAI1 leads to Smith-Magenis syndrome and obesity; therefore, its stable expression is a key asset for studying the transcriptional regulation of circadian rhythm and satiety. Genome integration ensures the target gene establishes a permanent presence in stable cell models. Rigorous quality control, including titer and sterility testing, ensures the repeatability and safety of your results.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: RAI1

Full Name: Retinoic acid induced 1

Gene Symbol: SMS; SMCR

Gene ID: 10743

RefSeq ID-1: NP_109590.3

RefSeq ID-2: NM_030665.3

Summary: RAI1 is a transcriptional regulator highly expressed in the brain. It is the primary gene responsible for Smith-Magenis Syndrome, located on chromosome 17. The protein is sensitive to retinoic acid and plays a vital role in neurodevelopment and the regulation of circadian rhythms. Patients with RAI1 mutations or deletions often exhibit severe obesity, disrupted sleep patterns, and behavioral changes, illustrating the gene's control over the neurological aspects of metabolic health.

AdipoUpX™ Human RAI1 MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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