AdipoUpX™ Human PRKAR1A MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PRKAR1A MMLV Particle (Overexpression) is a high-titer, ready-to-use MMLV retroviral particle professionally engineered for the stable overexpression of the human PRKAR1A gene. As a regulatory subunit of PKA, its stable integration provides a robust model for investigating cAMP signaling in lipolysis and endocrine function. Our vector system features a modular design for high flexibility. To ensure experimental safety, every batch undergoes comprehensive quality control, including titer, sterility, and mycoplasma detection.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: PRKAR1A

Full Name: Protein kinase cAMP-dependent type I regulatory subunit alpha

Gene Symbol: CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1; Prkar1alpha

Gene ID: 5573

RefSeq ID-1: NP_001263218.1

RefSeq ID-2: NM_001276289.2

Summary: The PRKAR1A gene encodes a critical regulatory subunit of the cAMP-dependent protein kinase (PKA) complex. By controlling the activation of PKA in response to cAMP levels, this protein influences diverse cellular processes, including DNA replication and nutrient metabolism. Genetic mutations in PRKAR1A are the primary cause of Carney complex and are linked to various endocrine tumors.

AdipoUpX™ Human PRKAR1A MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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