AdipoUpX™ Human PDK4 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PDK4 Lentiviral Particle (Overexpression) is professionally engineered for the long-lasting, stable overexpression of the human PDK4 gene. This kinase is a central regulator of the pyruvate dehydrogenase complex, making it a critical tool for studying the inhibition of glucose oxidation in insulin-resistant states. Integrating into the host genome, it allows for the creation of stable cell lines to investigate metabolic fuel switching. The product is ready-to-use and high-titer, with all particles undergoing rigorous internal quality control validation to ensure the highest experimental standards.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: PDK4

Full Name: Pyruvate dehydrogenase kinase 4

Gene ID: 5166

RefSeq ID-1: NP_002603.1

RefSeq ID-2: NM_002612.4

Summary: PDK4 encodes a mitochondrial kinase that inhibits the pyruvate dehydrogenase complex, regulating glucose oxidation and metabolic flexibility. By controlling fuel utilization in adipocytes and other tissues, PDK4 contributes to energy balance and obesity risk. Expression is regulated by insulin, glucocorticoids, and retinoic acid.

AdipoUpX™ Human PDK4 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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