AdipoUpX™ Human LPL MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human LPL MMLV Particle (Overexpression) is a high-titer MMLV particle designed for the efficient and stable overexpression of the human LPL gene. Lipoprotein lipase is central to the hydrolysis of triglycerides and the partitioning of fatty acids between muscles and fat. This MMLV system is a perfect tool for investigating lipid clearance mechanisms in establishing stable adipocyte or muscle cell models. The viral system features a modular design for high flexibility, and all products undergo rigorous QC screening, including titer and mycoplasma detection.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: LPL

Full Name: Lipoprotein lipase

Gene Symbol: LIPD; HDLCQ11

Gene ID: 4023

RefSeq ID-1: NP_000228.1

RefSeq ID-2: NM_000237.3

Summary: LPL encodes lipoprotein lipase, an enzyme expressed predominantly in the heart, muscle, and adipose tissue. LPL functions as an active homodimer and performs a dual critical role in lipid metabolism: acting as a triglyceride hydrolase (breaking down triglycerides in circulating lipoproteins) and serving as a ligand or bridging factor for receptor-mediated lipoprotein uptake. Severe genetic deficiencies in LPL result in Type I hyperlipoproteinemia, characterized by massively elevated triglyceride levels. Less severe LPL mutations are widely implicated in various common disorders of lipoprotein metabolism.

AdipoUpX™ Human LPL MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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