Product Overview
Description:
AdipoUpX™ Human INS MMLV Particle (Overexpression) is a ready-to-use retroviral vector particle professionally engineered for the stable overexpression of the human INS gene. By enabling the target gene to integrate into the host genome, this product allows researchers to establish reliable cell models for studying insulin synthesis and its systemic metabolic effects. It facilitates the investigation of glucose homeostasis and beta-cell function. All viral particle products undergo rigorous quality control (titer, sterility, and mycoplasma testing) to ensure the highest standards of safety and reproducibility.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.
Sterility Test:
The final viral preparation was confirmed sterile after testing.
Mycoplasma Test:
Mycoplasma contamination testing met all release specifications for all product lots.
Other QC:
We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.
Storage:
The product must be immediately transferred to -80 °C storage upon its arrival.
Stability:
For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.
Shipping Condition:
Our MMLV retroviral products are shipped using dry ice.
Handling Notes:
Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.
Target Profile
Gene Name:
INS
Full Name:
Insulin
Gene Symbol:
IDDM; ILPR; IRDN; IDDM1; IDDM2; PNDM4; MODY10
Summary:
The INS gene produces insulin, a central regulator of systemic glucose and lipid homeostasis. Following translation, the insulin precursor is sequentially processed to yield mature insulin, composed of two peptide chains connected by disulfide bonds, along with the co-secreted C-peptide. Insulin exerts its biological effects by binding to the insulin receptor, thereby promoting glucose uptake and metabolic signaling. Numerous INS mutations have been identified and are responsible for a range of metabolic disorders, including neonatal diabetes, MODY type 10, and hyperproinsulinemia. The INS locus partially overlaps with the INS-IGF2 read-through transcript, reflecting complex genomic organization.
