AdipoUpX™ Human BBS1 MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human BBS1 MMLV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human BBS1 gene. As a major component of the BBSome, it is critical for researching ciliary transport and its role in Bardet-Biedl Syndrome-related obesity. The MMLV vector integrates stably into the host genome, providing an ideal tool for in vitro functional validation in dividing cells. Product quality is ensured by rigorous purification and comprehensive QC screening against contaminants to ensure experimental repeatability.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: BBS1

Full Name: Bardet-Biedl syndrome 1

Gene Symbol: BBS2L2

Gene ID: 582

RefSeq ID-1: NP_078925.3

RefSeq ID-2: NM_024649.4

Summary: BBS1 encodes a protein that is a critical component of the BBSome, a large protein complex essential for the assembly, trafficking, and function of primary cilia. Mutations in this gene are responsible for the most common form of Bardet-Biedl Syndrome (BBS Type 1), a pleiotropic, recessive ciliopathy. BBS is characterized by a constellation of developmental and systemic defects affecting the entire body, notably including retinal degeneration, polydactyly, renal anomalies, learning disabilities, and truncal obesity, demonstrating the profound impact of ciliary dysfunction on development and metabolic regulation.

AdipoUpX™ Human BBS1 MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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