AdipoUpX™ Human ATG7 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ATG7 Lentiviral Particle (Overexpression) is a powerful gene delivery tool specifically designed for the stable overexpression of the human ATG7 gene. Autophagy is a critical regulator of adipocyte browning and mitochondrial quality control; genome integration allows for the establishment of stable cell lines to study these essential turnover mechanisms. All particles undergo rigorous quality control screening to ensure experimental reliability. Our modular design provides high flexibility for customization through various markers and labels.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: ATG7

Full Name: Autophagy related 7

Gene Symbol: GSA7; APG7L; SCAR31; APG7-LIKE

Gene ID: 10533

RefSeq ID-1: NP_001129503.2

RefSeq ID-2: NM_001136031.3

Summary: ATG7 is an essential enzyme for the process of autophagy, the cell's internal recycling system. It is required for the formation of autophagosomes, which digest damaged components to provide energy during metabolic stress. ATG7 is particularly vital during the differentiation of fat cells, where it helps remodel the cellular architecture to accommodate large lipid droplets.

AdipoUpX™ Human ATG7 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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