Sheep insulin (INS) ELISA kit (10-100 μIU/mL)

Cat. No.: 0126WXX-627

Assay Type: Quantitative sandwich ELISA

Target Species: Sheep

Assay Target: INS

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Sheep insulin (INS) ELISA kit (10-100 μIU/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Sheep insulin (INS) ELISA kit (10-100 μIU/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in sheep samples with a range of 10-100 μIU/mL and a minimum detectable dose (sensitivity) of <2 μIU/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes a streamlined quantitative sandwich enzyme immunoassay technique, featuring a microplate pre-coated with a capture antibody specific for INS. Standards and samples are pipetted into the wells and incubated simultaneously with a HRP-conjugated detection antibody to form a "sandwich" complex in a single step or sequential format. Following a wash step to remove all unbound reagents, a substrate solution is added to initiate color development, the intensity of which is directly proportional to the amount of INS initially bound to the plate. The reaction is halted, and the color intensity is measured, providing a rapid and precise determination of INS levels based on the resulting spectrophotometric data.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Sheep insulin (INS) ELISA kit (10-100 μIU/mL) is used to quantify INS in serum and plasma of sheep, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma

Detection Range: 10-100 μIU/mL

Sensitivity: <2 μIU/mL

Precision (Intra-assay): CV<15%

Precision (Inter-assay): CV<15%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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