Rat tumor necrosis factor receptor superfamily member 1B (TNF-RII) ELISA kit (15.625-1000 pg/mL)

Cat. No.: 0126WXX-1928

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: TNF-RII

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Rat tumor necrosis factor receptor superfamily member 1B (TNF-RII) ELISA kit (15.625-1000 pg/mL)

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Product Overview

Description: Rat tumor necrosis factor receptor superfamily member 1B (TNF-RII) ELISA kit (15.625-1000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of TNF-RII in rat samples with a range of 15.625-1000 pg/mL and a minimum detectable dose (sensitivity) of 9.375 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes sandwich enzyme-linked immunosorbent assay technology. An anti-rat TNF-RII antibody is pre-coated onto 96-well plates. A biotin-conjugated anti-rat TNF-RII antibody serves as the detection agent. Standards, samples, and the biotinylated detection antibody are introduced into the wells and washed with buffer. HRP-streptavidin is added, followed by a wash to remove unbound conjugates. TMB substrates are used to visualize the HRP enzymatic reaction, which produces a blue color that shifts to yellow upon adding the stop solution. The resulting yellow density is proportional to the rat TNF-RII concentration. The O.D. absorbance is read at 450 nm, and the concentration is calculated accordingly.

Background: TNF-RII, also known as TNFRSF1B or TNF-R2, is a transmembrane receptor on the cell surface that primarily binds to the pro-inflammatory cytokine tumor necrosis factor TNF-α and plays a crucial role in mediating its biological functions. Unlike TNF-RI, which primarily mediates apoptosis, TNF-RII signaling predominantly activates cell survival and proliferation signals by recruiting the TRAF protein family. In obesity research, TNF-α is a key inflammatory factor released from hypertrophic adipose tissue and is considered one of the primary drivers of insulin resistance. TNF-RII is recognized as mediating most of TNF-α's metabolic effects, particularly in immune cells and endothelial cells. Elevated TNF-RII activity correlates with chronic inflammation, adipose tissue remodeling, and impaired insulin signaling, positioning it as a potential target linking obesity-induced chronic inflammation to metabolic dysfunction.

Synonyms: CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; Soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-R-II; TNFR-II; TNFRSF1B; Tumor necrosis factor beta receptor; Tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor superfamily; member 1B; Tumor necrosis factor receptor type II

Applications: Rat tumor necrosis factor receptor superfamily member 1B (TNF-RII) ELISA kit (15.625-1000 pg/mL) is used to quantify TNF-RII in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of rat, providing data to support research in a wide range of areas, including inflammation and immunology, metabolism, obesity, and others.

Research Area: Inflammation and immunology; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 15.625-1000 pg/mL

Sensitivity: 9.375 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 85-105%

Storage: Store at -20°C (microplate; standard) and 2-8°C (other reagents).

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