Chicken perilipin 1 (PLIN1) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-1355

Assay Type: Quantitative competitive ELISA

Target Species: Chicken

Assay Target: PLIN1

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Chicken perilipin 1 (PLIN1) ELISA kit-Quantitative competitive

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Product Overview

Description: Chicken perilipin 1 (PLIN1) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PLIN1 in chicken samples with a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This PLIN1 ELISA kit utilizes the competitive enzyme immunoassay principle, featuring a monoclonal anti-PLIN1 antibody and a PLIN1-HRP conjugate. Samples and assay buffers are incubated alongside the PLIN1-HRP conjugate in the pre-sensitized plate for one hour. Because the antibody sites are limited, the endogenous PLIN1 in the sample competes directly with the HRP-conjugated PLIN1 for binding. Following the incubation, the plate is decanted and washed five times. A substrate for the HRP enzyme is then introduced to develop a blue product, which turns yellow upon the addition of the stop solution. The signal intensity, recorded at 450 nm, is inversely proportional to the concentration of PLIN1. Final concentrations are calculated by comparing the sample O.D. values against a generated standard curve.

Background: PLIN1 is an adipocyte-specific lipid-binding protein that localizes to lipid droplets and plays a central role in regulating lipid storage and mobilization. By coating lipid droplets, PLIN1 protects stored triacylglycerols from basal hydrolysis while enabling controlled lipolysis in response to hormonal stimulation through phosphorylation by cAMP-dependent protein kinase. This dynamic regulation of lipid breakdown is essential for maintaining adipocyte lipid homeostasis. Dysregulation or mutation of PLIN1 impairs normal lipolytic control and fat storage, contributing to abnormal adipose tissue function and is closely associated with obesity and related metabolic disorders, including familial partial lipodystrophy.

Synonyms: PERI; Lipid droplet associated protein

Applications: Chicken perilipin 1 (PLIN1) ELISA kit-Quantitative competitive is used to quantify PLIN1 in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of chicken, providing data to support research in a wide range of areas, including metabolic pathways, obesity, and others.

Research Area: Metabolic pathway; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 94-103%

Storage: Store at 2-8°C.

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