Porcine insulin (INS) ELISA kit (1.56-100 mIU/L)

Cat. No.: 0126WXX-608

Assay Type: Quantitative sandwich ELISA

Target Species: Porcine

Assay Target: INS

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Porcine insulin (INS) ELISA kit (1.56-100 mIU/L)

INQUIRY Datasheet MSDS

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Product Overview

Description: Porcine insulin (INS) ELISA kit (1.56-100 mIU/L) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in porcine samples with a range of 1.56-100 mIU/L and a minimum detectable dose (sensitivity) of 0.5 mIU/L. The kit is highly sensitive and easy to use.

Assay Principle: This double-antibody sandwich ELISA uses a monoclonal porcine INS capture antibody and a biotin-labeled polyclonal detection antibody. Samples and detection antibodies are added to the plate and washed with PBS or TBS, followed by the addition of avidin-peroxidase conjugates. TMB substrate facilitates color development, which turns yellow upon adding acid. The resulting color depth is positively correlated with the concentration of INS present in the samples.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Porcine insulin (INS) ELISA kit (1.56-100 mIU/L) is used to quantify INS in serum, plasma, cell culture supernatant, and organizations of porcine, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Organizations

Detection Range: 1.56-100 mIU/L

Sensitivity: 0.5 mIU/L

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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