Porcine insulin receptor substrate 1 (IRS-1) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-707

Assay Type: Quantitative competitive ELISA

Target Species: Porcine

Assay Target: IRS-1

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Porcine insulin receptor substrate 1 (IRS-1) ELISA kit-Quantitative competitive

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Product Overview

Description: Porcine insulin receptor substrate 1 (IRS-1) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of IRS-1 in porcine samples. The kit is highly sensitive and easy to use.

Assay Principle: The IRS-1 ELISA kit utilizes a competitive enzyme immunoassay technique. This method features a monoclonal anti-IRS-1 antibody and an IRS-1-HRP conjugate. Samples and assay buffers are incubated alongside the IRS-1-HRP conjugate in a pre-coated plate for sixty minutes. During this time, endogenous IRS-1 from the sample competes with the HRP-labeled conjugate for a finite number of antibody binding sites. Following the incubation, the wells are washed five times to remove unbound reagents. An HRP-specific substrate is then added to form a blue product, which turns yellow upon the addition of a stop solution. The resulting color intensity, measured at 450 nm, is inversely proportional to the IRS-1 concentration. A standard curve is plotted to relate the optical density (O.D.) to the concentration of the standards, and the IRS-1 concentration for each sample is interpolated from this curve.

Background: IRS-1 is a key adaptor protein that plays a central role in insulin signaling. When insulin binds to its receptor on the cell surface, the receptor is activated and phosphorylates tyrosine residues on IRS-1. This process transforms IRS-1 into a signaling “relay station,” enabling it to bind with multiple downstream signaling proteins such as phosphoinositide 3-kinase (PI3K). IRS-1 transmits insulin signals from the cell membrane into the cell interior, thereby initiating a series of vital biological responses, including promoting glucose transport and glycogen synthesis. Studying the function and defects of IRS-1 is crucial for understanding the pathological processes underlying obesity.

Synonyms: IRS1

Applications: Porcine insulin receptor substrate 1 (IRS-1) ELISA kit-Quantitative competitive is used to quantify IRS-1 in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of porcine, providing data to support research in a wide range of areas, including endocrinology, obesity, etc.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Limit: 1.0 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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