Rat peroxisome proliferator-activated receptor α (PPARα) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-1492

Assay Type: Quantitative competitive ELISA

Target Species: Rat

Assay Target: PPARα

Size:

Rat peroxisome proliferator-activated receptor α (PPARα) ELISA kit-Quantitative competitive

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Rat peroxisome proliferator-activated receptor α (PPARα) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PPARα in rat samples with a minimum detectable dose (sensitivity) of 1.0 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This PPARα ELISA kit applies a competitive enzyme immunoassay technique utilizing a polyclonal anti-PPARα antibody and a PPARα-HRP conjugate. Samples and buffer are incubated with the conjugate in a pre-coated plate for one hour. After five wash cycles, an HRP substrate is added to generate color. The reaction is stopped, and the absorbance is measured at 450 nm. The color intensity is inversely proportional to the PPARα concentration due to competitive binding for the limited antibody sites.

Background: PPAR-alpha, a member of the NR1 nuclear hormone receptor subfamily located at chromosomal position 22q13.31, serves as a master ligand-activated transcription factor and a cornerstone regulator of lipid homeostasis in obesity research. Primarily localized within the nucleus, its transactivation activity—requiring heterodimerization with RXRA and being antagonized by NR2C2—is triggered by various endogenous ligands such as 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC), satiety-regulating lipids like oleylethanolamide, and exogenous peroxisome proliferators. Beyond its fundamental role in activating the peroxisomal beta-oxidation pathway through genes such as ACOX1 and P450, PPAR-alpha orchestrates a complex physiological network including fatty acid transport, lipoprotein metabolism, and the negative regulation of appetite and glycolysis. Its multifaceted molecular functions—ranging from zinc ion binding to steroid hormone-mediated signaling—integrate critical biological processes such as insulin stimulus response, gluconeogenesis, and circadian rhythm regulation, making it an essential target for understanding and intervening in metabolic disorders and chronic obesity.

Synonyms: Peroxisome proliferator activated receptor alpha; NR1C1; PPAR-A; HPPAR; Nuclear receptor subfamily 1 group C member 1; PPARa; PPARA; PPAR-alpha

Applications: Rat peroxisome proliferator-activated receptor α (PPARα) ELISA kit-Quantitative competitive is used to quantify PPARα in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of rat, providing data to support research in a wide range of areas, including signal transduction, developmental science, obesity, and others.

Research Area: Signal transduction; Developmental science; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 1.0 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 92-101%

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out