Rat peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.312-20 ng/mL)

Cat. No.: 0126WXX-1460

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: PPARα

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Rat peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.312-20 ng/mL)

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Product Overview

Description: Rat peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.312-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PPARα in rat samples with a range of 0.312-20 ng/mL and a minimum detectable dose (sensitivity) of 0.115 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific to PPARα. Standards or samples are added to the appropriate wells along with a biotin-conjugated detection antibody specific to PPARα. Subsequently, avidin conjugated to HRP is added to each well and incubated to form an immune complex. Following the addition of TMB substrate solution, only wells containing the PPARα complex will exhibit a color change. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution, and the absorbance is measured spectrophotometrically at a wavelength of 450 nm ± 10 nm. The concentration of PPARα in the samples is determined by comparing the optical density (O.D.) of the samples to the standard curve.

Background: PPAR-alpha, a member of the NR1 nuclear hormone receptor subfamily located at chromosomal position 22q13.31, serves as a master ligand-activated transcription factor and a cornerstone regulator of lipid homeostasis in obesity research. Primarily localized within the nucleus, its transactivation activity—requiring heterodimerization with RXRA and being antagonized by NR2C2—is triggered by various endogenous ligands such as 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC), satiety-regulating lipids like oleylethanolamide, and exogenous peroxisome proliferators. Beyond its fundamental role in activating the peroxisomal beta-oxidation pathway through genes such as ACOX1 and P450, PPAR-alpha orchestrates a complex physiological network including fatty acid transport, lipoprotein metabolism, and the negative regulation of appetite and glycolysis. Its multifaceted molecular functions—ranging from zinc ion binding to steroid hormone-mediated signaling—integrate critical biological processes such as insulin stimulus response, gluconeogenesis, and circadian rhythm regulation, making it an essential target for understanding and intervening in metabolic disorders and chronic obesity.

Synonyms: Peroxisome proliferator activated receptor alpha; NR1C1; PPAR-A; HPPAR; Nuclear receptor subfamily 1 group C member 1; PPARa; PPARA; PPAR-alpha

Applications: Rat peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.312-20 ng/mL) is used to quantify PPARα in tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids of rat, providing data to support research in a wide range of areas, including signal transduction, developmental science, obesity, and others.

Research Area: Signal transduction; Developmental science; Obesity

Specification

Sample Type: Tissue homogenates; Cell lysates; Cell culture supernatants; Other biological fluids

Detection Range: 0.312-20 ng/mL

Sensitivity: 0.115 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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