Mouse uncoupling protein 3 (UCP3) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-2078

Assay Type: Quantitative competitive ELISA

Target Species: Mouse

Assay Target: UCP3

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Mouse uncoupling protein 3 (UCP3) ELISA kit-Quantitative competitive

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Product Overview

Description: Mouse uncoupling protein 3 (UCP3) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of UCP3 in mouse samples with a minimum detectable dose (sensitivity) of 1.0 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This UCP3 ELISA kit utilizes a competitive enzyme immunoassay format featuring a polyclonal anti-UCP3 antibody and a UCP3-HRP conjugate. The test sample and buffer are incubated with the HRP-labeled conjugate in a pre-sensitized plate for sixty minutes. Because the specific binding sites on the polyclonal antibody are finite, the UCP3 in the sample competes with the UCP3-HRP conjugate for these positions. After five wash cycles, an HRP substrate is added to develop a blue product that turns yellow after the reaction is halted with the stop solution. The signal intensity at 450 nm is inversely proportional to the UCP3 concentration, which is then interpolated from a standard curve relating O.D. to standard concentrations.

Background: UCP3 is a key member of the UCP family, functioning as a transporter located in the inner mitochondrial membrane. Its primary role is to create a proton leakage pathway, allowing protons to bypass the ATP synthase and thereby decouple the oxidative phosphorylation process. This decoupling results in energy that would otherwise be used for ATP synthesis being dissipated as heat rather than stored in chemical bonds. In obesity research, UCP3 serves as a key regulator of thermogenesis and systemic energy balance, exhibiting high expression primarily in skeletal muscle. Studies indicate that elevated UCP3 levels may contribute to increased basal metabolic rate, enhanced fatty acid oxidation, and resistance to weight gain, positioning it as a potential therapeutic target for obesity treatment and improved insulin sensitivity.

Synonyms: SLC25A9; Mitochondrial uncoupling protein 3; UCP 3; Solute carrier family 25 member 9; Uncoupling protein 3; mitochondrial

Applications: Mouse uncoupling protein 3 (UCP3) ELISA kit-Quantitative competitive is used to quantify UCP3 in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of mouse, providing data to support research in a wide range of areas, including metabolism, mitochondrial function, oxidative stress, exercise physiology, energy balance, obesity, and others.

Research Area: Metabolism; Mitochondrial function; Oxidative stress; Exercise physiology and energy balance; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 1.0 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 92-101%

Storage: Store at 2-8°C.

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