Rat peroxisome proliferator-activated receptor gamma (PPARγ) ELISA kit (0.05-20 ng/mL)

Cat. No.: 0126WXX-1539

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: PPARγ

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Rat peroxisome proliferator-activated receptor gamma (PPARγ) ELISA kit (0.05-20 ng/mL)

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Product Overview

Description: Rat peroxisome proliferator-activated receptor gamma (PPARγ) ELISA kit (0.05-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PPARγ in rat samples with a range of 0.05-20 ng/mL and a minimum detectable dose (sensitivity) of 0.01 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The plate is pre-coated with a rat PPARγ antibody. Rat PPARγ in the sample binds to the immobilized antibodies, followed by the binding of a biotinylated rat PPARγ detection antibody and streptavidin-HRP. After incubation and washing, a substrate solution is introduced. Color develops in direct proportion to the amount of rat PPARγ. The reaction is terminated with an acidic stop solution, and the absorbance is measured at 450 nm.

Background: PPAR-gamma (Peroxisome Proliferator-Activated Receptor Gamma), a prominent member of the nuclear hormone receptor superfamily located at chromosomal position 3p25, functions as a master ligand-dependent transcription factor essential for adipogenesis, lipid homeostasis, and glucose metabolism. Preferentially expressed in adipocytes, vascular smooth muscle cells, and macrophages, this DNA-binding nuclear receptor operates as a heterodimer with the retinoid X receptor and interacts with coactivators such as NCOA6 to significantly enhance the transcription of target genes. Its biological activity, which is modulated by MAP kinase-mediated phosphorylation and insulin stimuli, governs critical processes including white and brown fat cell differentiation, long-chain fatty acid transport, and the regulation of systemic insulin sensitivity. Beyond its primary metabolic roles, PPAR-gamma serves as a receptor for fatty acids and hypolipidemic drugs, influencing diverse physiological pathways ranging from the negative regulation of inflammation and smooth muscle cell proliferation to the orchestration of circadian rhythms and blood pressure. Consequently, dysregulation or genetic variation in PPAR-gamma is fundamentally linked to the pathogenesis of obesity, non-insulin-dependent diabetes mellitus, and familial partial lipodystrophy, underscoring its pivotal role as a central hub in metabolic health and disease.

Synonyms: PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone receptor; Nuclear receptor subfamily 1 group C member 3; PPARg; Peroxisome proliferator activated receptor gamma; PPAR-γ; Peroxisome proliferator-activated receptor γ

Applications: Rat peroxisome proliferator-activated receptor gamma (PPARγ) ELISA kit (0.05-20 ng/mL) is used to quantify PPARγ in serum, plasma, cell culture supernatants, ascites, tissue homogenates, and other biological fluids of rat, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, cardiovascular biology, developmental science, bone metabolism, obesity, and others.

Research Area: Metabolic pathway; Endocrinology; Cardiovascular biology; Developmental science; Bone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; ascites; Tissue homogenates; Other biological fluids

Detection Range: 0.05-20 ng/mL

Sensitivity: 0.01 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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