Rat peptide YY (PYY) ELISA kit (15.6-1000 pg/mL)

Cat. No.: 0126WXX-1674

Assay Type: Quantitative competitive ELISA

Target Species: Rat

Assay Target: PYY

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Rat peptide YY (PYY) ELISA kit (15.6-1000 pg/mL)

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Product Overview

Description: Rat peptide YY (PYY) ELISA kit (15.6-1000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PYY in rat samples with a range of 15.6-1000 pg/mL and a minimum detectable dose (sensitivity) of 8.14 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This ELISA is based on the competitive binding enzyme immunoassay technique. The provided microplate is pre-coated with an antibody specific to PYY. During the reaction, PYY in the sample or standard competes with a fixed amount of biotin-labeled PYY for binding sites on the immobilized monoclonal antibody. After washing the plate, avidin-HRP is added and incubated. TMB substrate is then introduced to develop color. The reaction is stopped with sulphuric acid, and the O.D. is measured at 450 nm ± 2 nm, where the intensity is inversely proportional to the PYY concentration.

Background: PYY (Peptide YY), a critical gut peptide and member of the NPY family located at chromosomal position 17q21.1, serves as a fundamental regulator in the physiological landscape of obesity and digestive homeostasis. Secreted into the extracellular region as two distinct isoforms through alternative splicing, this neuropeptide hormone exerts its biological influence primarily through G-protein coupled receptor signaling pathways to modulate eating behavior and appetite regulation. Its multifaceted role in the gastrointestinal tract includes the inhibition of exocrine pancreatic secretion, the mediation of vasoconstrictory actions, and the suppression of jejunal and colonic motility, thereby slowing digestion to enhance satiety. Beyond its classical endocrine functions in feeding behavior, PYY is also implicated in broader cellular processes such as cell proliferation and cytoskeleton organization.

Synonyms: Peptide YY-like

Applications: Rat peptide YY (PYY) ELISA kit (15.6-1000 pg/mL) is used to quantify PYY in rat samples, providing data to support research in a wide range of areas, including neuroscience, hepatology, obesity, and others.

Research Area: Neuro science; Hepatology; Obesity

Specification

Sample Type: Rat samples

Detection Range: 15.6-1000 pg/mL

Sensitivity: 8.14 pg/mL

Precision (Intra-assay): CV≤5.2%

Precision (Inter-assay): CV≤10.4%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 0.88

Storage: Store at -20°C.

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