Rat pancreatic lipase (PL) ELISA kit (0.312-20 ng/mL)

Cat. No.: 0126WXX-1340

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: PL

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Rat pancreatic lipase (PL) ELISA kit (0.312-20 ng/mL)

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Product Overview

Description: Rat pancreatic lipase (PL) ELISA kit (0.312-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PL in rat samples with a range of 0.312-20 ng/mL and a minimum detectable dose (sensitivity) of <0.06 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay utilizes a typical double-sandwich ELISA technique. The microtiter plate is pre-coated with a Rat PL monoclonal antibody, and the detection antibody is a biotin-labeled polyclonal antibody. Samples and the biotinylated detection antibody are added to the wells and washed with PBS or TBS. Subsequently, avidin-peroxidase conjugates are added. Following a thorough wash, TMB substrate is used for coloration. The blue catalytic product turns yellow under the action of acid, with the color depth being positively correlated with the target factors in the sample.

Background: PL, a key member of the lipase gene family located at chromosomal position 10q26.1, is a specialized carboxyl esterase (EC 3.1.1.3) specifically expressed in the pancreas and secreted into the extracellular region. This hydrolase is essential for the efficient digestion of dietary fats, specifically driving the hydrolysis of insoluble, emulsified triglycerides into absorbable components. Beyond its primary role in lipid catabolism and cholesterol absorption, PNLIP is involved in retinoid metabolic processes and is the central molecular driver in Pancreatic Lipase Deficiency, making it a fundamental target for understanding gastrointestinal lipid digestion and its systemic impact on energy balance.

Synonyms: PNLIP; PTL; Lipase; pancreatic; Pancreatic triacylglycerol lipase

Applications: Rat pancreatic lipase (PL) ELISA kit (0.312-20 ng/mL) is used to quantify PL in serum, plasma, cell culture supernatant of rat, providing data to support research in a wide range of areas, including enzyme and kinase, metabolic pathway, Infection immunity, hepatology, obesity, and others.

Research Area: Enzyme & Kinase; Metabolic pathway; Infection immunity; Hepatology; Obesity

Specification

Sample Type: Rat serum; Plasma; Cell culture supernatant

Detection Range: 0.312-20 ng/mL

Sensitivity: <0.06 ng/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 70-110%

Storage: Store at 2-8°C.

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