Products
- Anti-Obesity Compound Library
- GPCR/G Protein-Targeted Compounds
- Immunology/Inflammation-Targeted Compounds
- JAK/STAT-Targeted Compounds
- MAPK-Targeted Compounds
- Membrane Transporter/Ion Channel-Targeted Compounds
- Metabolism-Targeted Compounds
- NF-κB-Targeted Compounds
- Microbiology/Virology-Targeted Compounds
- Neuronal Signaling-Targeted Compounds
- PI3K/Akt/mTOR-Targeted Compounds
- Oxidation-reduction-Targeted Compounds
- Proteases/Proteasome-Targeted Compounds
- Stem Cells/Wnt-Targeted Compounds
- Tyrosine Kinase/Adaptors-Targeted Compounds
- Ubiquitin-Targeted Compounds
Online Inquiry
Monkey growth hormone (GH) ELISA kit-Quantitative sandwich
Cat. No.:
0126WXX-117
Assay Type:
Quantitative sandwich ELISA
Target Species:
Monkey
Assay Target:
GH
Size:
Add to basket
Product Overview
Description:
Monkey growth hormone (GH) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GH in monkey samples with a range of 2.5-50 ng/mL and a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.
Assay Principle:
The GH ELISA kit utilizes a competitive enzyme immunoassay technique involving a monoclonal anti-GH antibody and a GH-HRP conjugate. The sample and assay buffer are incubated together with the GH-HRP conjugate in a pre-coated microplate for one hour. During this period, endogenous GH from the sample and the GH-HRP conjugate compete for a limited number of binding sites on the anti-GH antibody. After incubation, the wells are decanted and washed five times to remove unbound materials. The wells are then incubated with an HRP enzyme substrate, triggering a reaction that forms a blue-colored complex. Finally, a stop solution is added to terminate the reaction, shifting the color to yellow. The absorbance is measured spectrophotometrically at 450 nm. The color intensity is inversely proportional to the GH concentration; as more sites are occupied by GH from the sample, fewer sites remain available for the GH-HRP conjugate. A standard curve is plotted to relate the optical density (OD) to the concentration of the standards, allowing the GH concentration in each sample to be interpolated.
Background:
Growth hormone (GH) is a crucial endocrine hormone that plays a key role in growth regulation and metabolic control. It primarily promotes bodily growth by stimulating the liver and other tissues to secrete insulin-like growth factor-1 (IGF-1), while also enhancing amino acid uptake and protein synthesis. Deficiencies in GH can lead to growth hormone deficiency disorders, such as dwarfism. Research indicates that GH induces lipolysis through direct lipolytic effects in adipose tissue and influences obesity to a certain extent.
Synonyms:
GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary growth hormone; Growth hormone, normal
Applications:
Monkey growth hormone (GH) ELISA kit is used to quantify GH in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of monkey, providing data to support research in a wide range of areas, including developmental biology, obesity, endocrinology, neuro science, metabolism, and others.
Research Area:
Developmental biology; Obesity; Endocrinology; Neuro science; Metabolism
Specification
Sample Type:
Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate
Detection Range:
2.5-50 ng/mL
Sensitivity:
0.1 ng/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Recovery:
92-101%
Storage:
Store at 2-8°C.