Human cathepsin A (CTSA) ELISA kit

Cat. No.: OB0625WXX-497

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: CTSA

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Product Overview

Description: Human cathepsin A (CTSA) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CTSA in human with a range of 31.2-1000 pg/mL and a specificity of 5.0 pg/mL.

Assay Principle: Solid-phase antibody is made from purified human CTSA antibody, then HRP-labeled CTSA and CTSA antibody are added to the wells so that the reaction became an antibody-antigen-antibody-enzyme complex, washed completely. Then TMB substrate solution is added, and the TMB substrate changed to a blue color catalyzed by the HRP enzyme. Then sulfuric acid is added to terminate the reaction, and the change of the color is measured spectrophotometrically at 450 nm. The color change is measured spectrophotometrically at 450 nm. The color change is measured spectrophotometrically at 450 nm. The concentration of CTSA in the sample is then determined by comparing the OD of the sample with the standard curve.

Background: CTSA is a lysosomal serine carboxypeptidase. It binds with the lysosomal enzymes beta-galactosidase and neuraminidase, forming a high-molecular-weight multimeric complex, and has a protective role for their stability and activity.

Synonyms: Protective protein for beta-galactosidase; Galactosialidosis; Carboxypeptidase C; Carboxypeptidase L; CTS-A; GLB2; GSL; NGBE; PPCA; PPGB; EC 3.4.16.5

EC NO.: 3.4.16.5

Applications: Human cathepsin A (CTSA) ELISA kit is used to quantify CTSA in serum, plasma, tissue homogenate, feces, and urine samples of humans, providing data to support a wide range of studies.

Research Area: Metabolism; Homeostasis; Obesity research

Specification

Sample Type: Serum; Plasma; Tissue homogenate; Feces; Urine

Detection Range: 31.2-1000 pg/mL

Sensitivity: 5.0 pg/mL

Precision (Intra-assay): CV<15%

Precision (Inter-assay): CV<15%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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