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Rat lipopolysaccharide binding protein (LBP) ELISA kit (1.88-120 ng/mL)
Cat. No.:
0126WXX-775
Assay Type:
Quantitative sandwich ELISA
Target Species:
Rat
Assay Target:
LBP
Size:
Add to basket
Product Overview
Description:
Rat lipopolysaccharide binding protein (LBP) ELISA kit (1.88-120 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LBP in rat samples with a range of 1.88-120 ng/mL and a minimum detectable dose (sensitivity) of 1.13 ng/mL. The kit is highly sensitive and easy to use.
Assay Principle:
The microplate provided is pre-coated with an antibody specific to rat LBP. Standards or samples are added to the wells, followed by the sequential addition of a biotinylated detection antibody for rat LBP and an avidin-HRP conjugate. After washing away unbound components, a substrate solution is added to produce a blue color that turns yellow upon adding the stop solution. The O.D. value, measured at 450 nm ± 2 nm, is directly proportional to the concentration of rat LBP.
Background:
LBP is a secreted protein primarily present in the extracellular space. Its core function is to bind bacterial lipopolysaccharide (LPS), a glycolipid found on the outer membrane of all Gram-negative bacteria. LBP is significant in obesity research because it plays a pivotal role in the link between inflammation, gut microbiota, and metabolic health. By studying LBP's mechanisms of action and its level changes, scientists can better understand how obesity leads to systemic inflammation and insulin resistance, potentially developing therapies targeting LBP or its associated signaling pathways.
Synonyms:
LPS-binding protein
Applications:
Rat lipopolysaccharide binding protein (LBP) ELISA kit (1.88-120 ng/mL) is used to quantify LBP in serum, plasma, and other biological fluids of rat, providing data to support research in a wide range of areas, including Infection immunity, obesity, etc.
Research Area:
Infection immunity; Obesity
Specification
Sample Type:
Serum; Plasma; Other biological fluids
Detection Range:
1.88-120 ng/mL
Sensitivity:
1.13 ng/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 4°C.
