Rat lipopolysaccharide binding protein (LBP) ELISA kit (0.312-20 ng/mL)

Cat. No.: 0126WXX-777

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: LBP

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Rat lipopolysaccharide binding protein (LBP) ELISA kit (0.312-20 ng/mL)

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Product Overview

Description: Rat lipopolysaccharide binding protein (LBP) ELISA kit (0.312-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LBP in rat samples with a range of 0.312-20 ng/mL and a minimum detectable dose (sensitivity) of 0.137 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate is pre-coated with an antibody specific to LBP. Standards or samples are added to the wells with a biotin-conjugated detection antibody specific for LBP. Next, avidin-conjugated HRP is introduced to the microplate wells and incubated. Upon the addition of TMB substrate, color development occurs exclusively in wells containing the target LBP complex. The reaction is terminated with a sulphuric acid solution, and the color change is measured at a wavelength of 450 nm ± 10 nm. The final concentration of LBP in the specimens is determined by mapping the O.D. results against the standard curve.

Background: LBP is a secreted protein primarily present in the extracellular space. Its core function is to bind bacterial lipopolysaccharide (LPS), a glycolipid found on the outer membrane of all Gram-negative bacteria. LBP is significant in obesity research because it plays a pivotal role in the link between inflammation, gut microbiota, and metabolic health. By studying LBP's mechanisms of action and its level changes, scientists can better understand how obesity leads to systemic inflammation and insulin resistance, potentially developing therapies targeting LBP or its associated signaling pathways.

Synonyms: LPS-binding protein

Applications: Rat lipopolysaccharide binding protein (LBP) ELISA kit (0.312-20 ng/mL) is used to quantify LBP in serum, plasma, and other biological fluids of rat, providing data to support research in a wide range of areas, including Infection immunity, obesity, etc.

Research Area: Infection immunity; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 0.312-20 ng/mL

Sensitivity: 0.137 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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