Porcine fibroblast growth factor 21 (FGF21) ELISA kit (15.6-1000 pg/mL)

Cat. No.: 0126WXX-03

Assay Type: Quantitative sandwich ELISA

Target Species: Porcine

Assay Target: FGF21

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Porcine fibroblast growth factor 21 (FGF21) ELISA kit (15.6-1000 pg/mL)

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Product Overview

Description: Porcine fibroblast growth factor 21 (FGF21) ELISA kit (15.6-1000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FGF21 in porcine samples with a range of 15.6-1000 pg/mL and a minimum detectable dose (sensitivity) of 5.9 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes the sandwich enzyme immunoassay principle. The provided microtiter plate is pre-immobilized with an antibody specific to FGF21. Standards or samples are added to the wells with a biotin-conjugated antibody specific to FGF21. Next, avidin-HRP is added and incubated. Upon the addition of TMB substrate, color development occurs only in wells containing the FGF21 sandwich complex. The enzymatic reaction is terminated with sulphuric acid, and the color change is measured at 450 nm ± 10 nm. The FGF21 concentration is determined by comparing the sample O.D. to the standard curve.

Background: FGF21 is an endocrine hormone secreted by multiple tissues, particularly the liver. It belongs to the fibroblast growth factor family but functions differently from traditional growth factors. FGF21 is primarily induced during stress or fasting states, acting as a crucial metabolic regulator that influences the body's energy balance, glucose homeostasis, and fatty acid metabolism. FGF21 plays a pivotal role in the onset, progression, and treatment of obesity. Its analogues have emerged as novel therapeutic agents and are being investigated for their potential in anti-obesity interventions.

Synonyms: Fgf21; FGF-21

Applications: Porcine fibroblast growth factor 21 (FGF21) ELISA kit (15.6-1000 pg/mL) is used to quantify FGF21 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids of porcine, providing data to support research in a wide range of areas, including cytokine, metabolism, obesity, and others.

Research Area: Cytokine; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 15.6-1000 pg/mL

Sensitivity: 5.9 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 88-96%

Storage: Store at 2-8°C.

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