Rat insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (3.125-200 ng/mL)

Cat. No.: 0126WXX-492

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: IGFBP-2

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Rat insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (3.125-200 ng/mL)

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Product Overview

Description: Rat insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (3.125-200 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of IGFBP-2 in rat samples with a range of 3.125-200 ng/mL and a minimum detectable dose (sensitivity) of 1.34 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes a sandwich enzyme immunoassay technique. A microplate is pre-coated with a capture antibody specific to IGFBP-2. Standards or samples are introduced into the wells along with a biotin-conjugated detection antibody. This process forms a "sandwich" complex, effectively capturing any IGFBP-2 present in the specimen. After incubation, an avidin-HRP conjugate is added to each well. A final wash step removes all unbound components before the addition of a TMB substrate solution. The resulting colorimetric change is measured spectrophotometrically at 450 nm ± 10 nm after the enzymatic reaction is terminated with a sulfuric acid solution. The concentration of IGFBP-2 in the samples is then determined by comparing their absorbance (O.D.) to a standard curve.

Background: IGFBP-2 is a multifunctional molecule that plays a crucial role in human growth, metabolism, and cellular processes by regulating the biological activity of insulin-like growth factors (IGFs). Primarily through binding to IGF proteins, IGFBP-2 prolongs their half-life in the body and modulates their interactions with cell surface receptors, thereby inhibiting or enhancing the pro-growth effects of IGFs. The role of IGFBP-2 in obesity has garnered increasing attention. Its reduced levels are closely associated with obesity, dyslipidemia, and insulin resistance.

Synonyms: IBP-2; IGF-binding protein 2; IGFBP2; BP2, IBP2; Insulin-Like Growth Factor Binding Protein 2, 36kDa; Insulin-Like Growth Factor-Binding Protein 2

Applications: Rat insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (3.125-200 ng/mL) is used to quantify IGFBP-2 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids of rat, providing data to support research in a wide range of areas, including immunity, endocrinology, signal transduction, obesity, and others.

Research Area: Immunity; Endocrinology; Signal transduction; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 3.125-200 ng/mL

Sensitivity: 1.34 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).

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