Rat endothelial lipase (EL) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-904

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: EL

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Rat endothelial lipase (EL) ELISA kit (0.156-10 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Rat endothelial lipase (EL) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of EL in rat samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.086 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The ELISA analytical biochemical technique is based on EL antibody-EL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EL antigen targets in samples.

Background: EL is a lipase primarily expressed in vascular endothelial cells. Its link to obesity stems from its potent influence on HDL metabolism: Similar to HL, EL primarily hydrolyzes phospholipids within HDL. Elevated EL activity leads to a significant reduction in HDL levels, impairing cholesterol reverse transport. Given that low HDL levels are a common feature of elevated cardiovascular risk in obese and metabolic syndrome patients, EL activity is considered a key enzyme regulating dyslipidemia and obesity-related complications.

Synonyms: Lipase G, Endothelial type; Endothelial lipase isoform 2; EDL; LIPG; Endothelial cell-derived lipase

EC NO.: 3.1.1.3

Applications: Rat endothelial lipase (EL) ELISA kit (0.156-10 ng/mL) is used to quantify EL in serum, plasma, whole blood, tissue, saliva, urine, feces, and other fluids of rat, providing data to support research in a wide range of areas, including enzymes and kinases, metabolism, obesity, etc.

Research Area: Enzymes and kinases; Metabolism; obesity

Specification

Sample Type: Serum; Plasma; Whole blood; Other fluid; Tissue; Saliva; Urine; Feces

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.086 ng/mL

Precision (Intra-assay): CV≤4.5%

Precision (Inter-assay): CV≤5.9%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 0.91

Storage: Store at -20°C.

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