Rat cathepsin S (CTSS) ELISA kit (15.6-1000 pg/mL)

Cat. No.: OB0625WXX-544

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: CTSS

Size:

Rat cathepsin S (CTSS) ELISA kit (15.6-1000 pg/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Rat cathepsin S (CTSS) ELISA kit (15.6-1000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CTSS in rat with a range of 15.6-1000 pg/mL and a specificity of <3.9 pg/mL.

Assay Principle: An antibody specific for CTSS has been pre-coated on a microtiter plate. Standards and samples are pipetted into the wells, and any CTSS is bound by the immobilized antibody. After removing unbound material, a biotin-conjugated antibody specific for CTSS is added to the wells. After washing, avidin-conjugated horseradish peroxidase (HRP) is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color is developed proportionally to the amount of CTSS bound in the first step. The color development is stopped, and the intensity of the color is measured.

Background: CTSS is an important member of the cysteine protease family. As a potent mammalian elastase, it can degrade various extracellular components, including fibronectin, laminin, elastin, and collagen. CTSS is abnormally expressed in various disease states, such as obesity and atherosclerosis, which makes it a biomarker and a potential therapeutic target. Studies have found that CTSS inhibitors may be a possible strategy for treating obesity.

Synonyms: CTS-S; EC 3.4.22.27

EC NO.: 3.4.22.27

Applications: Rat cathepsin S (CTSS) ELISA kit (15.6-1000 pg/mL) is used to quantify CTSS in serum, plasma, tissue homogenates, cell lysates samples of rat, providing data to support a wide range of studies.

Research Area: Obesity research; Immune response; Signaling pathway; Protein degradation

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates

Detection Range: 15.6-1000 pg/mL

Sensitivity: <3.9 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out