Rat C-reactive protein (CRP) ELISA kit (3-25 μg/mL)

Cat. No.: OB0625WXX-465

Assay Type: Quantitative ELISA

Target Species: Rat

Assay Target: CRP

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Rat C-reactive protein (CRP) ELISA kit (3-25 μg/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Rat C-reactive protein (CRP) ELISA kit (3-25 μg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CRP in rats with a range of 3-25 μg/mL.

Assay Principle: The canine serum to be tested is diluted to 1:500 and reacts with pneumococcal C polysaccharide coated on specially treated microtiter wells. After appropriate incubation, the wells are washed to remove unreacted serum proteins, and then enzyme-labeled goat anti-dog CRP (conjugate) is added to react with and label the antigen-antibody complex. After further incubation, the wells are washed again to remove unreacted conjugate. Color development is initiated by the addition of urea peroxide substrate with TMB as the chromogen. A blue color indicates a positive reaction, while a negative reaction is colorless or has a trace of blue. The reaction is interrupted with a stopping solution, which changes the blue color of the positive reaction to yellow. The negative reaction remains colorless or slightly yellow. The color intensity (absorbance) is read at 450nm using a spectrophotometer or ELISA reader.

Background: CRP is an acute-phase protein of hepatic origin, whose circulating concentrations rise in response to inflammation. It is considered a key plasma protein that can bind to Leptin, and its potential role in regulating leptin's function in obesity is crucial.

Synonyms: Pentraxin-related; pentraxin 1; C-RP; PTX1

Applications: Rat C-reactive protein (CRP) ELISA kit (3-25 μg/mL) is used to quantify CRP in rat samples, providing data to support a wide range of studies.

Research Area: Inflammatory reaction; Obesity research

Specification

Sample Type: Rat samples

Detection Range: 3-25 μg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C.

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