Rat adrenocorticotropic hormone (ACTH) ELISA kit (31.2-2000 pg/mL)

Cat. No.: OB0525WXX-190

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: ACTH

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Rat adrenocorticotropic hormone (ACTH) ELISA kit (31.2-2000 pg/mL)

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Product Overview

Description: Rat adrenocorticotropic hormone (ACTH) ELISA kit (31.2-2000 pg/mL) is a ready-to-use ELISA kit for analyzing rat ACTH levels with a detection range of 31.2-2000 pg/mL and a sensitivity of up to 12 pg/mL.

Assay Principle: This experiment uses the double sandwich ELISA technique, and the ELISA kit provided is a typical double sandwich ELISA kit. The pre-coated antibody is a rat ACTH monoclonal antibody, and the detection antibody is a biotin-labeled polyclonal antibody. Samples and biotin-labeled antibodies are added to the ELISA plate wells and rinsed with PBS or TBS. Avidin-peroxidase conjugate is then sequentially added to the ELISA wells; the reactants are thoroughly rinsed with PBS or TBS and then colored with TMB substrate. TMB turns blue under the catalysis of peroxidase and finally turns yellow in the presence of acid. The shade of color is positively correlated with the detection factor in the sample.

Background: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase in body weight beyond the limitations of skeletal and physical requirements, as a result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency, and red hair.

Synonyms: ACTH; Corticotropin; Adrenocorticotropin; Adrenocorticotropic hormone; Pomc1; Pomc-1; Beta-LPH; AlphaMSH; beta-MSH; Gamma-LPH; Alpha-MSH; Gamma-MSH

Applications: Rat adrenocorticotropic hormone (ACTH) ELISA kit (31.2-2000 pg/mL) is designed for the in vitro quantitative analysis of ACTH levels in rat serum, plasma, cell culture supernatant samples.

Research Area: Hormone research; Metabolites; Signaling pathway; Glucose homeostasis; Regulation of appetite; Obesity

Specification

Sample Type: Rat serum; Plasma; Cell culture supernatant

Detection Range: 31.2-2000 pg/mL

Sensitivity: Up to 12 pg/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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