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Rat adiponectin (ADP) ELISA kit (0.156-10 ng/mL, 0.039 ng/mL)
Cat. No.:
OB0525WXX-279
Assay Type:
Quantitative ELISA
Target Species:
Rat
Assay Target:
ADP
Size:
Add to basket
Product Overview
Description:
Rat adiponectin (ADP) ELISA kit (0.156-10 ng/mL, 0.039 ng/mL) is a ready-to-use ELISA kit for analyzing rat ADP levels with a detection range of 0.156-10 ng/mL and a sensitivity of 0.039 ng/mL.
Assay Principle:
The microtiter plates provided in this kit are pre-coated with an antibody specific for ADP. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated ADP-specific antibody preparation, and HRP-conjugated avidin is added to each microtiter plate well and incubated. A TMB (3,3'5, 5'tetramethylbenzidine) substrate solution is then added to each well. Only wells containing ADP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of ADP in the sample is then determined by comparing the OD of the sample to a standard curve.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Synonyms:
ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin
Applications:
Rat adiponectin (ADP) ELISA kit (0.156-10 ng/mL, 0.039 ng/mL) is designed for the in vitro quantitative analysis of ADP levels in rat serum, plasma, cell culture supernates, and tissue homogenate samples.
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Specification
Sample Type:
Serum; Plasma; Cell culture supernates; Tissue homogenates
Detection Range:
0.156-10 ng/mL
Sensitivity:
0.039 ng/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.
