Rabbit lipopolysaccharide binding protein (LBP) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-773

Assay Type: Quantitative competitive ELISA

Target Species: Rabbit

Assay Target: LBP

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Rabbit lipopolysaccharide binding protein (LBP) ELISA kit-Quantitative competitive

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Product Overview

Description: Rabbit lipopolysaccharide binding protein (LBP) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LBP in rabbit samples with a minimum detectable dose (sensitivity) of 0.1 μg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The LBP ELISA kit applies a competitive enzyme immunoassay technique utilizing an anti-LBP antibody and an LBP-HRP conjugate. Samples and buffer are incubated with the conjugate in a pre-coated plate for one hour. After five wash cycles, an HRP substrate is added to form a blue product, turning yellow upon the addition of the stop solution. The color intensity at 450 nm is inversely proportional to the LBP concentration because sample LBP competes with the LBP-HRP conjugate for a limited number of antibody binding sites. Results are interpolated from a standard curve.

Background: LBP is a secreted protein primarily present in the extracellular space. Its core function is to bind bacterial lipopolysaccharide (LPS), a glycolipid found on the outer membrane of all Gram-negative bacteria. LBP is significant in obesity research because it plays a pivotal role in the link between inflammation, gut microbiota, and metabolic health. By studying LBP's mechanisms of action and its level changes, scientists can better understand how obesity leads to systemic inflammation and insulin resistance, potentially developing therapies targeting LBP or its associated signaling pathways.

Synonyms: LPS-binding protein

Applications: Rabbit lipopolysaccharide binding protein (LBP) ELISA kit-Quantitative competitive is used to quantify LBP in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of rabbit, providing data to support research in a wide range of areas, including Infection immunity, obesity, etc.

Research Area: Infection immunity; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 0.1 μg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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