Porcine uncoupling protein 3 (UCP3) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-2082

Assay Type: Quantitative competitive ELISA

Target Species: Porcine

Assay Target: UCP3

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Porcine uncoupling protein 3 (UCP3) ELISA kit-Quantitative competitive

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Product Overview

Description: Porcine uncoupling protein 3 (UCP3) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of UCP3 in porcine samples with a range of 0.5-10 ng/mL and a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This UCP3 ELISA kit applies a competitive enzyme immunoassay technique utilizing a monoclonal anti-UCP3 antibody and a UCP3-HRP conjugate. Samples and assay buffer are incubated with the HRP conjugate in a pre-coated plate for one hour. Following the incubation period, the wells are decanted and washed five times. An HRP enzyme substrate is added, forming a blue-colored complex that turns yellow once the stop solution is added. The color intensity, measured at 450 nm, is inversely proportional to the UCP3 concentration, as sample-derived UCP3 and the conjugate compete for a limited number of monoclonal antibody binding sites. A standard curve is plotted to interpolate the specific UCP3 concentration in each sample.

Background: UCP3 is a key member of the UCP family, functioning as a transporter located in the inner mitochondrial membrane. Its primary role is to create a proton leakage pathway, allowing protons to bypass the ATP synthase and thereby decouple the oxidative phosphorylation process. This decoupling results in energy that would otherwise be used for ATP synthesis being dissipated as heat rather than stored in chemical bonds. In obesity research, UCP3 serves as a key regulator of thermogenesis and systemic energy balance, exhibiting high expression primarily in skeletal muscle. Studies indicate that elevated UCP3 levels may contribute to increased basal metabolic rate, enhanced fatty acid oxidation, and resistance to weight gain, positioning it as a potential therapeutic target for obesity treatment and improved insulin sensitivity.

Synonyms: SLC25A9; Mitochondrial uncoupling protein 3; UCP 3; Solute carrier family 25 member 9; Uncoupling protein 3; mitochondrial

Applications: Porcine uncoupling protein 3 (UCP3) ELISA kit-Quantitative competitive is used to quantify UCP3 in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of porcine, providing data to support research in a wide range of areas, including metabolism, mitochondrial function, oxidative stress, exercise physiology, energy balance, obesity, and others.

Research Area: Metabolism; Mitochondrial function; Oxidative stress; Exercise physiology and energy balance; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 0.5-10 ng/mL

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 94-103%

Storage: Store at 2-8°C.

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