Porcine leptin receptor (LEPR) ELISA kit (0.31-20 ng/mL)

Cat. No.: 0126WXX-852

Assay Type: Quantitative sandwich ELISA

Target Species: Porcine

Assay Target: LEPR

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Porcine leptin receptor (LEPR) ELISA kit (0.31-20 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Pig leptin receptor (LEPR) ELISA kit-Quantitative sandwich is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEPR in porcine samples. The kit is highly sensitive and easy to use.

Assay Principle: An antibody specific for LEPR has been pre-immobilized on a microplate. Standards and samples are introduced into the wells, where the capture antibody binds any present LEPR. After washing away unbound components, a biotin-conjugated detection antibody specific for LEPR is added. Following another wash, streptavidin-conjugated HRP is added. After a final wash to clear unbound enzyme, a substrate solution is introduced, allowing color to develop in proportion to the bound LEPR. The process concludes with the addition of a stop solution and absorbance measurement.

Background: LEPR, as a peptide hormone receptor, is responsible for receiving leptin signals. When leptin binds to LEPR, it initiates a series of intracellular cascades, including the positive regulation of the JAK-STAT signaling pathway and the positive regulation of the PI3K signaling pathway. These pathways are crucial for translating leptin signals into biological responses, such as appetite suppression and metabolic regulation. The relationship between LEPR and obesity is paramount. When leptin functions as the “satiety hormone,” LEPR acts as the “satiety switch” in the brain and other tissues. Without functional receptors, leptin signals cannot be received regardless of how high leptin concentrations become.

Synonyms: CD295; LEPR; OBR; LR

Applications: Porcine leptin receptor (LEPR) ELISA kit (0.31-20 ng/mL) is used to quantify LEPR in serum, plasma, and other biological fluids of porcine, providing data to support research in a wide range of areas, including signal transduction, CD & adhesion molecule, metabolic pathway, endocrinology, obesity, etc.

Research Area: Signal transduction; CD & Adhesion molecule; Metabolic pathway; Endocrinology; Obesity