Porcine hormone-sensitive lipase (HSL) ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-894

Assay Type: Quantitative sandwich ELISA

Target Species: Porcine

Assay Target: HSL

Size:

Porcine hormone-sensitive lipase (HSL) ELISA kit-Quantitative sandwich

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Porcine hormone-sensitive lipase (HSL) ELISA kit-Quantitative sandwich is an ELISA-based in vitro research tool designed specifically for the quantitative detection of HSL in porcine samples. The kit is highly sensitive and easy to use.

Assay Principle: This assay utilizes a two-site sandwich ELISA to quantify HSL in samples. An antibody specific for HSL is pre-coated onto the microplate. Standards and samples are pipetted into the wells, and any HSL present is captured by the immobilized antibody. After washing away unbound substances, a biotin-conjugated detection antibody specific for HSL is introduced. Subsequently, streptavidin-conjugated HRP is added. Following a final wash to remove unbound enzymes, a substrate solution is added for color development, which is proportional to the amount of HSL bound. The development is halted by a stop solution, and the color intensity is recorded.

Background: HSL is the key enzyme for fat breakdown. Belonging to the “GDXG” lipase family, this enzyme primarily hydrolyzes stored triacylglycerols into free fatty acids in adipose tissue and the heart. This process represents the rate-limiting step of lipolysis, controlling the overall speed of fat breakdown. HSL can be rapidly activated by catecholamine hormones (such as epinephrine) through a cyclic adenosine monophosphate (cAMP)-dependent phosphorylation process, while insulin regulates its dephosphorylation and inactivation. Due to its functional characteristics, HSL has become a central subject in obesity research, particularly in the regulation of fat storage and release.

Synonyms: LHS; LIPE; Lipase, hormone sensitive; EC 3.1.1.79

EC NO.: 3.1.1.79

Applications: Pig hormone-sensitive lipase (HSL) ELISA kit-Quantitative sandwich is used to quantify HSL in serum, plasma, and other biological fluids of pig, providing data to support research in a wide range of areas, including metabolic pathway, obesity, etc.

Research Area: Metabolic pathway; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out