Rat glucagon-like peptide 2 (GLP-2) ELISA kit (15.6-1000 pg/mL)

Cat. No.: 0126WXX-228

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: GLP-2

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Rat glucagon-like peptide 2 (GLP-2) ELISA kit (15.6-1000 pg/mL)

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Product Overview

Description: Rat glucagon-like peptide 2 (GLP-2) ELISA kit (15.6-1000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GLP-2 in rat samples with a range of 15.6-1000 pg/mL and a minimum detectable dose (sensitivity) of 5 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay utilizes a double-sandwich ELISA technique. The microplate is pre-coated with a rat GLP-1 monoclonal antibody, and the detection antibody is a biotin-labeled polyclonal antibody. Samples and the detection antibody are added to the wells and washed with PBS or TBS. Avidin-peroxidase conjugates and TMB substrate are then added in sequence. The enzymatic reaction produces a blue product that turns yellow under acidic conditions. The color intensity is positively correlated with the GLP-1 concentration in the sample.

Background: GLP-2, as an incretin, primarily functions to promote intestinal growth and nutrient absorption. Recent studies have revealed that GLP-2's association with obesity primarily manifests through its regulatory effects on intestinal barrier function and the gut microbiome. By improving gut health, GLP-2 may indirectly influence metabolism. Furthermore, ongoing research is exploring the potential applications of GLP-2 and its analogues in regulating fat metabolism and energy expenditure, offering new avenues for obesity treatment.

Synonyms: Glp2; GLP2

Applications: Rat glucagon-like peptide 2 (GLP-2) ELISA kit (15.6-1000 pg/mL) is used to quantify GLP-2 in rat serum, plasma, cell culture supernatant, and organizations, providing data to support research in a wide range of areas, including metabolism, endocrinology, cardiovascular biology, obesity, and others.

Research Area: Metabolism; Endocrinology; Cardiovascular biology; Obesity

Specification

Sample Type: Rat serum; Plasma; Cell culture supernatant; Organizations

Detection Range: 15.6-1000 pg/mL

Sensitivity: 5 pg/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 70-110%

Storage: Store at 2-8°C.

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