Mouse NAD-dependent protein deacetylase sirtuin-1 (SIRT1) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-1807

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: SIRT1

Size:

Mouse NAD-dependent protein deacetylase sirtuin-1 (SIRT1) ELISA kit (0.156-10 ng/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Mouse NAD-dependent protein deacetylase sirtuin-1 (SIRT1) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of SIRT1 in mouse samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.055 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The provided microtiter plate is pre-coated with a capture antibody specific to SIRT1. Standards or samples are pipetted into the appropriate wells along with a biotin-conjugated antibody preparation specific for SIRT1. Following this, avidin-conjugated HRP is added to each microplate well and incubated. After the addition of TMB substrate solution, color change will only be observed in wells where SIRT1 is present as part of the sandwich complex. The reaction is terminated by a sulphuric acid solution, and the optical density is recorded at 450 nm ± 10 nm. The concentration of SIRT1 in each sample is calculated relative to the O.D. values of the standard curve.

Background: Sirtuin 1 (SIRT1) is an NAD+-dependent protein deacetylase that links cellular energy status to transcriptional regulation, modulating processes such as metabolism, autophagy, apoptosis, and cell cycle control. SIRT1 deacetylates a variety of transcription factors and coregulators, influencing gene expression in liver, muscle, and adipose tissue, and participates in chromatin silencing through energy-dependent complexes. By sensing NAD+/NADH ratios, SIRT1 integrates metabolic signals and contributes to the regulation of glucose and lipid homeostasis, as well as cellular responses to stress and nutrient availability.

Synonyms: SIR2L1; Silent mating type information regulation 2 homolog 1; SIR2-like protein 1; SirtT1 75 kDa fragment; Sirtuin 1

Applications: Mouse NAD-dependent protein deacetylase sirtuin-1 (SIRT1) ELISA kit (0.156-10 ng/mL) is used to quantify SIRT1 in serum, plasma, tissue homogenates, and cell lysates, and other biological fluids of mouse, providing data to support research in a wide range of areas, including endocrinology, obesity, and others.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Other biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.055 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate; wash buffer; stop solution) and -20°C (others).

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out