Mouse leptin (LEP) ELISA kit (62.5-4000 pg/mL)

Cat. No.: 0126WXX-822

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: LEP

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Mouse leptin (LEP) ELISA kit (62.5-4000 pg/mL)

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Product Overview

Description: Mouse leptin (LEP) ELISA kit (62.5-4000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEP in mice samples with a range of 62.5-4000 pg/mL and a minimum detectable dose (sensitivity) of <10 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is based on sandwich ELISA technology. An anti-LEP polyclonal antibody is pre-coated onto 96-well plates. Standards, samples, and a biotin-conjugated anti-LEP polyclonal detection antibody are incubated in the wells, followed by a wash step and the addition of an avidin-biotin-peroxidase complex. TMB substrate generates a blue product that turns yellow upon acidification. The density of the yellow color is proportional to the amount of LEP captured, measured at 450 nm.

Background: LEP is a hormone that plays a crucial role in regulating body fat. Secreted by fat cells, it functions as part of a signaling pathway that controls the extent of fat storage in the human body. This protein belongs to the leptin family and possesses dual functions as both a growth factor and a hormone. LEP's primary functions include: regulating food intake, controlling energy expenditure, participating in glucose homeostasis regulation, modulating adipocyte differentiation, and influencing fatty acid metabolism. Obesity research frequently focuses on deciphering the mechanisms of leptin resistance and developing countermeasures, including the development of drugs that enhance brain sensitivity to leptin or the exploration of alternative pathways for regulating appetite and energy balance. Understanding leptin-mediated signaling pathways is central to this research.

Synonyms: OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

Applications: Mouse leptin (LEP) ELISA kit (62.5-4000 pg/mL) is used to quantify LEP in serum, plasma, whole blood, tissue, saliva, urine, feces, and other fluids of mouse, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, cardiovascular biology, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Cardiovascular biology; Obesity

Specification

Sample Type: Serum; Plasma; Whole blood; Other fluid; Tissue; Saliva; Urine; Feces

Detection Range: 62.5-4000 pg/mL

Sensitivity: <10 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C.

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