Mouse glucagon (GCG) ELISA kit (31.25-2000 pg/mL)

Cat. No.: 0126WXX-70

Assay Type: Quantitative competitive ELISA

Target Species: Mouse

Assay Target: GCG

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Mouse glucagon (GCG) ELISA kit (31.25-2000 pg/mL)

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Product Overview

Description: Mouse glucagon (GCG) ELISA kit (31.25-2000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GCG in mouse samples with a range of 31.25-2000 pg/mL and a minimum detectable dose (sensitivity) of 18.75 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is based on a competitive ELISA method, where the final signal intensity is inversely proportional to the concentration of the target antigen. The microplate is pre-coated with the target antigen. Antigen in the standard or sample competes with the pre-coated antigen for the binding sites of a biotin-labeled detection antibody. After washing, HRP-Streptavidin is added to each well. Subsequently, a TMB substrate solution is introduced, and the enzymatic reaction is stopped with sulfuric acid. The resulting color intensity is measured spectrophotometrically at 450 nm and interpolated from a standard curve.

Background: Glucagon is a hormone secreted by A cells of the islets of Langerhans, playing a pivotal role in glucose metabolism and homeostasis. Its primary function is to regulate blood glucose levels by promoting gluconeogenesis and inhibiting glycolysis. The dynamic equilibrium between glucagon and insulin is crucial for maintaining healthy metabolism. The detection of glucagon and analysis of its associated pathways provide key insights into understanding and treating obesity.

Synonyms: GLP1; GLP2; GRPP; Glicentin-related polypeptide; Glucagen; Oxyntomodulin; Incretin hormone; GC

Applications: Mouse glucagon (GCG) ELISA kit (31.25-2000 pg/mL) is used to quantify GCG in serum, plasma, cell culture supernatant, cell, tissue lysate, and other liquid samples of mouse, providing data to support research in a wide range of areas, including endocrinology, obesity, metabolism, and others.

Research Area: Endocrinology; Obesity; Metabolism

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell; Tissue lysate; Other liquid samples

Detection Range: 31.25-2000 pg/mL

Sensitivity: 18.75 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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