Mouse glucagon (GCG) ELISA kit (0.625-40 pg/mL)

Cat. No.: 0126WXX-71

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: GCG

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Mouse glucagon (GCG) ELISA kit (0.625-40 pg/mL)

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Product Overview

Description: Mouse glucagon (GCG) ELISA kit (0.625-40 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GCG in mouse samples with a range of 0.625-40 pg/mL and a minimum detectable dose (sensitivity) of 0.2 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: An antibody specific for GCG is pre-coated onto a microplate as the capture antibody. Standards and samples are added, allowing any GCG present to bind. After removing unbound substances, a biotin-conjugated antibody is added to form a sandwich. Following a wash, a streptavidin-HRP conjugate is introduced to bind the biotinylated antibody. Finally, a substrate solution is added; color develops in direct proportion to the amount of GCG bound in the initial capture step. The reaction is stopped, and the color intensity is measured to determine the GCG concentration.

Background: Glucagon is a hormone secreted by A cells of the islets of Langerhans, playing a pivotal role in glucose metabolism and homeostasis. Its primary function is to regulate blood glucose levels by promoting gluconeogenesis and inhibiting glycolysis. The dynamic equilibrium between glucagon and insulin is crucial for maintaining healthy metabolism. The detection of glucagon and analysis of its associated pathways provide key insights into understanding and treating obesity.

Synonyms: GLP1; GLP2; GRPP; Glicentin-related polypeptide; Glucagen; Oxyntomodulin; Incretin hormone; GC

Applications: Mouse glucagon (GCG) ELISA kit (0.625-40 pg/mL) is used to quantify GCG in serum, plasma, and other biological fluids of mouse, providing data to support research in a wide range of areas, including endocrinology, obesity, metabolism, and others.

Research Area: Endocrinology; Obesity; Metabolism

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 0.625-40 pg/mL

Sensitivity: 0.2 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Reaction Time: 1.5 h

Storage: Store at 2-8°C.

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