Mouse fibroblast growth factor 21 (FGF21) high sensitive ELISA kit (15.6-1000 pg/mL)

Cat. No.: OB0625WXX-891

Assay Type: Quantitative competitive ELISA

Target Species: Mouse

Assay Target: FGF21

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Mouse fibroblast growth factor 21 (FGF21) high sensitive ELISA kit (15.6-1000 pg/mL)

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Product Overview

Description: Mouse fibroblast growth factor 21 (FGF21) high sensitive ELISA kit (15.6-1000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FGF21 in mouse with a range of 15.6-1000 pg/mL and a specificity of 6.0 pg/mL.

Assay Principle: A monoclonal antibody specific for high-sensitivity FGF21 has been pre-coated on a microtiter plate. Competitive inhibition of the reaction between biotin-labeled High Sensitivity FGF21 and unlabeled high-sensitivity FGF21 (standard or sample) and the pre-coated high-sensitivity FGF21-specific antibody occurs. After incubation, unbound conjugates are washed away. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter well and incubated. The amount of bound HRP is inversely proportional to the concentration of high-sensitivity FGF21 in the sample. Upon addition of the substrate solution, the intensity of color development is inversely proportional to the concentration of highly sensitive FGF21 in the sample.

Background: FGF21 is a member of the FGF family and is the primary endogenous agonist for the FGF21 receptor. It is involved in various physiological processes and is associated with obesity. Studies have found that treatment with FGF21 analogues can normalize glucose utilization in obese mice and have shown some anti-obesity effects.

Synonyms: FGF-21; Fgf21

Applications: Mouse fibroblast growth factor 21 (FGF21) high sensitive ELISA kit (15.6-1000 pg/mL) is used to quantify FGF21 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples of mouse, providing data to support a wide range of studies.

Research Area: Cytokine; Metabolism; Obesity research

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 15.6-1000 pg/mL

Sensitivity: 6.0 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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