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Mouse fatty-acid amide hydrolase (FAAH) ELISA kit
Cat. No.:
OB0625WXX-802
Assay Type:
Quantitative competitive ELISA
Target Species:
Mouse
Assay Target:
FAAH
Size:
Add to basket
Product Overview
Description:
Mouse fatty-acid amide hydrolase (FAAH) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FAAH in mouse with a specificity of 1.0 ng/mL.
Assay Principle:
The assay sample and buffer are incubated with the FAAH-HRP conjugate in a precoated plate for one hour. At the end of the incubation period, the wells are poured out and washed five times. The wells are then incubated with HRP enzyme substrate. The products of the enzyme-substrate reaction form a blue color complex. Finally, the reaction is stopped by adding a stop solution, which turns the solution yellow. The color intensity is measured spectrophotometrically at 450 nm on a microplate reader. The color intensity is inversely proportional to the FAAH concentration because the FAAH in the sample competes with the FAAH-HRP conjugate for the binding site of the anti-FAAH antibody. Since there are a limited number of binding sites, as more sites are occupied by FAAH in the sample, there are fewer sites left to bind to the FAAH-HRP conjugate. A standard curve is plotted between color intensity (O.D.) and standard concentration. The FAAH concentration in each sample is derived from this standard curve.
Background:
FAAH degrades bioactive fatty acid amides into corresponding acids, thereby terminating the signaling function of these molecules. It participates in neural signaling pathways involved in regulating feeding and is associated with obesity.
Synonyms:
FAAH-1; Anandamide amidohydrolase 1; Oleamide hydrolase 1; EC 3.5.1.99
EC NO.:
3.5.1.99
Applications:
Mouse fatty-acid amide hydrolase (FAAH) ELISA kit is an important tool for the quantitative detection of FAAH in various biological samples (serum, plasma, cell culture supernatants, body fluid, tissue homogenate) of mice.
Research Area:
Enzyme and Kinase; Fatty acid metabolism; Obesity research
Specification
Sample Type:
Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate
Sensitivity:
1.0 ng/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.
