Mouse estrogen receptor α (ERα) ELISA kit (0.312-20 ng/mL, 0.146 ng/mL)

Cat. No.: OB0625WXX-729

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: ERα

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Mouse estrogen receptor α (ERα) ELISA kit (0.312-20 ng/mL, 0.146 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Mouse estrogen receptor α (ERα) ELISA kit (0.312-20 ng/mL, 0.146 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of ERα in mouse with a range of 0.312-20 ng/mL and a specificity of 0.146 ng/mL.

Assay Principle: The microtiter plates provided in this kit are pre-coated with an ERα-specific antibody. Standards or samples are then added to the appropriate microtiter plate wells along with the biotin-conjugated ERα-specific antibody preparation. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microplate well and incubated. Upon addition of TMB substrate solution, only wells containing ERα, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The OD of the sample is then compared to a standard curve to determine the concentration of ERα in the sample.

Background: ERα, a member of the steroid receptor superfamily, contains highly conserved DNA-binding and ligand-binding domains. It is activated by the sex hormone estrogen and is involved in the physiological development and function of various organ systems.

Synonyms: Estrogen receptor alpha; ESR1; ERa; NR3A1; EC 3.1.1.3

Applications: Mouse estrogen receptor α (ERα) ELISA kit (0.312-20 ng/mL, 0.146 ng/mL) is used to quantify ERα in tissue homogenates, cell lysates, and other biological fluid samples of mouse, providing data to support a wide range of studies.

Research Area: Endocrinology

Specification

Sample Type: Tissue homogenates; Cell lysates; Other biological fluids

Detection Range: 0.312-20 ng/mL

Sensitivity: 0.146 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).

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