Human glucocorticoid receptor-beta (GR-β) ELISA kit

Cat. No.: 0126WXX-295

Assay Type: Quantitative ELISA

Target Species: Human

Assay Target: GR-β

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Human glucocorticoid receptor-beta (GR-β) ELISA kit

INQUIRY Datasheet MSDS

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Product Overview

Description: Human glucocorticoid receptor-beta (GR-β) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GR-β in human samples. The kit is highly sensitive and easy to use.

Assay Principle: This immunoenzymatic assay for the quantitative measurement of GR-β employs a polyclonal anti-GR-β antibody and a GR-β-HRP conjugate. Samples and buffer are incubated with the conjugate in a pre-coated plate for one hour, allowing endogenous GR-β to compete for limited binding sites. Following five wash cycles, the wells are incubated with an HRP substrate. The resulting blue product shifts to yellow after adding a stop solution. Absorbance is measured spectrophotometrically at 450 nm. Since color intensity is inversely proportional to the GR-β concentration, the levels in each sample are determined by interpolating their O.D. values against a concurrent standard curve.

Background: GR-β is a splice isoform of the glucocorticoid receptor (GR). Unlike the physiologically active GR-α we are familiar with, GR-β cannot bind glucocorticoids and thus cannot mediate the classical glucocorticoid signaling pathway. Its primary function is believed to be the negative regulation of GR-α activity. It can form heterodimers with GR-α, preventing GR-α from entering the cell nucleus and binding to DNA, thereby inhibiting GR-α-mediated gene transcription. Consequently, GR-β is regarded as an endogenous inhibitor or antagonist of glucocorticoid action. Changes in GR-β levels may be associated with diseases such as obesity.

Synonyms: GRb; GR-beta

Applications: Human glucocorticoid receptor-beta (GR-β) ELISA kit is used to quantify GR-β in cell culture fluid, body fluid, tissue homogenate, serum, and blood plasma of human, providing data to support research in a wide range of areas, including signal transduction, endocrinology, hormone metabolism, obesity, and others.

Research Area: Signal transduction; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Cell culture fluid; Body fluid; Tissue homogenate; Serum; Blood plasma

Detection Range: 0.5-10 ng/mL

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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