Mouse alpha-ketoglutarate-dependent dioxygenase (FTO) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-34

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: FTO

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Mouse alpha-ketoglutarate-dependent dioxygenase (FTO) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Mouse alpha-ketoglutarate-dependent dioxygenase (FTO) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FTO in mouse samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.085 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The ELISA analytical biochemical technique is based on FTO antibody-FTO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FTO antigen targets in samples.

Background: FTO is a crucial dioxygenase primarily responsible for repairing alkylated DNA and RNA through oxidative demethylation. It exhibits the highest activity toward single-stranded RNA containing 3-methyluracil and requires molecular oxygen, α-ketoglutarate, and iron as cofactors. Beyond its role in nucleic acid repair, FTO participates in regulating systemic metabolic rate, energy expenditure, and energy homeostasis, thereby influencing body composition and adipose tissue accumulation. Defects in this protein are associated with various disorders, including growth retardation, developmental delays, and premature mortality. The relationship between FTO and obesity represents a highly significant research area, given that FTO is currently one of the strongest known genetic associates of obesity. Research indicates that FTO influences body weight by regulating energy balance and fat accumulation. Its mechanisms involve modulating appetite and satiety signaling pathways, as well as influencing lipolysis and lipogenesis processes. Specific FTO gene variants have been demonstrated to be significantly associated with higher BMI and obesity risk.

Synonyms: GDFD; IFEX9; ALKBH9; BMIQ14; KIAA1752; Fat mass and obesity-associated protein; FTO, alpha-ketoglutarate dependent dioxygenase; EC 1.14.11.-

EC NO.: 1.14.11.-

Applications: Mouse alpha-ketoglutarate-dependent dioxygenase (FTO) ELISA kit (0.156-10 ng/mL) is used to quantify FTO in mouse samples, providing data to support research in a wide range of areas, including enzyme & kinase, obesity, and others.

Research Area: Enzyme & kinase; Obesity

Specification

Sample Type: Mouse samples

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.085 ng/mL

Precision (Intra-assay): <=8.1%

Precision (Inter-assay): <=10.5%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 1.01

Storage: Store at -20°C.

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