Rat plasminogen activator inhibitor-1 (PAI-1) ELISA kit (0.312-20 ng/mL)

Cat. No.: 0126WXX-1224

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: PAI-1

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Rat plasminogen activator inhibitor-1 (PAI-1) ELISA kit (0.312-20 ng/mL)

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Product Overview

Description: Rat plasminogen activator inhibitor-1 (PAI-1) ELISA kit (0.312-20 ng/mL, 0.121 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PAI-1 in rat samples with a range of 0.312-20 ng/mL and a minimum detectable dose (sensitivity) of 0.121 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay utilizes the Sandwich enzyme immunoassay technique. The microtiter plate is pre-coated with an antibody specific to PAI1. Standards or samples are introduced into the wells with a biotin-conjugated detection antibody for PAI1. Subsequently, avidin-conjugated HRP is added and incubated. After the TMB substrate is introduced, wells containing the target complex will undergo a color change. The reaction is stopped with sulphuric acid, and the O.D. is measured at 450 nm ± 10 nm. The concentration of PAI1 is then calculated by mapping results against the standard curve.

Background: As the primary physiological inhibitor of tissue-type plasminogen activator and urokinase-type plasminogen activator, PAI-1 exerts its effects by suppressing fibrinolysis and proteolysis. Its elevation is associated not only with thrombosis and fibrosis but also with obesity and insulin resistance.

Synonyms: SERPINE1; Endothelial plasminogen activator inhibitor

Applications: Rat plasminogen activator inhibitor-1 (PAI-1) ELISA kit (0.312-20 ng/mL, 0.121 ng/mL) is used to quantify PAI-1 in Plasma of rat, providing data to support research in a wide range of areas, including metabolism, fibrosis, thrombosis, obesity, etc.

Research Area: Metabolism; Fibrosis; Thrombosis; Obesity

Specification

Sample Type: Plasma

Detection Range: 0.312-20 ng/mL

Sensitivity: 0.121 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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