Mouse agouti-related protein (AGRP) ELISA kit (15.625-1000 pg/mL, 9.375 pg/mL)

Cat. No.: OB0525WXX-395

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: AGRP

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Mouse agouti-related protein (AGRP) ELISA kit (15.625-1000 pg/mL, 9.375 pg/mL)

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Product Overview

Description: Mouse agouti-related protein (AGRP) ELISA kit (15.625-1000 pg/mL, 9.375 pg/mL) is a ready-to-use ELISA kit for analyzing mouse AGRP levels with a detection range of 15.625-1000 pg/mL and a sensitivity of 9.375 pg/mL.

Assay Principle: The kit is based on a sandwich ELISA technique. The capture antibody is pre-coated on a 96-well plate. Biotin-conjugated antibodies are used as detection antibodies. Standards, test samples, and biotin-conjugated detection antibodies are subsequently added to the wells and washed with buffer. HRP-streptavidin is added, and the unbound conjugate is washed away with washing solution. TMB substrate is used to visualize the HRP enzyme reaction. TMB produces a blue product catalyzed by HRP, which turns yellow upon addition of an acidic termination solution. The density of the yellow color is proportional to the amount of target sample captured in the plate. The concentration of the target can be calculated by reading the absorbance at 450nm with a microplate reader.

Background: AGRP plays a role in body weight homeostasis, is involved in the control of eating behavior through the central melanocortin system, and acts as an inverse agonist of MC3R and MC4R, thereby inhibiting their constitutive activity. Genetic variants in AGRP may be responsible for obesity.

Synonyms: Agouti-related peptide; ASIP2

Applications: Mouse agouti-related protein (AGRP) ELISA kit (15.625-1000 pg/mL, 9.375 pg/mL) is designed for the in vitro quantitative analysis of AGRP levels in mouse serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Research Area: Hormone research; Eating behavior; Signaling pathway; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 15.625-1000 pg/mL

Sensitivity: 9.375 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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