Mouse adrenocorticotropic hormone (ACTH) ELISA kit (5-600 ng/L)

Cat. No.: OB0525WXX-185

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: ACTH

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Mouse adrenocorticotropic hormone (ACTH) ELISA kit (5-600 ng/L)

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Product Overview

Description: Mouse adrenocorticotropic hormone (ACTH) ELISA kit (5-600 ng/L) is a ready-to-use ELISA kit for analyzing mouse ACTH levels with a detection range of 5-600 ng/L and a sensitivity of 3.13 ng/L.

Assay Principle: The plates are pre-coated with mouse ACTH antibody. ACTH added to the sample binds to the antibody coated on the wells. Biotinylated mouse ACTH antibody is then added and binds to ACTH in the sample. Streptavidin-HRP is then added and binds to the biotinylated ACTH antibody. After incubation, unbound streptavidin-HRP is washed out. The substrate solution is then added, and the color is developed in proportion to the mouse ACTH content. The reaction is terminated by the addition of an acidic termination solution, and the absorbance is measured at 450 nm.

Background: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase in body weight beyond the limitations of skeletal and physical requirements, as a result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency, and red hair.

Synonyms: ACTH; Corticotropin; Adrenocorticotropin; Adrenocorticotropic hormone; Pomc1; Pomc-1; Beta-LPH; AlphaMSH; beta-MSH; Gamma-LPH; Alpha-MSH; Gamma-MSH

Applications: Mouse adrenocorticotropic hormone (ACTH) ELISA kit (5-600 ng/L) is designed for the in vitro quantitative analysis of ACTH levels in mouse serum, plasma, cell culture supernates, cell lysates, and tissue homogenate samples.

Research Area: Hormone research; Metabolites; Signaling pathway; Glucose homeostasis; Regulation of appetite; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernates; Cell lysates; Tissue homogenates

Detection Range: 5-600 ng/L

Sensitivity: 3.13 ng/L

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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