Human 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) ELISA kit (30-9000 ng/L)

Cat. No.: OB0525WXX-002

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: 11β-HSD1

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Human 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) ELISA kit (30-9000 ng/L)

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Product Overview

Description: Human 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) ELISA kit (30-9000 ng/L) is a ready-to-use ELISA kit for analyzing human 11β-HSD1 levels with a detection range of 30-9000 ng/L and a sensitivity of 15.19 ng/L. This kit has high sensitivity and excellent specificity.

Assay Principle: The plate has been pre-coated with human 11β-HSD1 antibody. The 11β-HSD1 added to the sample binds to the antibody coated on the wells. Biotinylated human 11β-HSD1 antibody is then added and binds to 11β-HSD1 in the sample. Streptavidin-HRP is then added to bind to the biotinylated 11β-HSD1 antibody. After incubation, unbound streptavidin-HRP is washed out. Substrate solution is then added, and color formation is proportional to the amount of human 11β-HSD1. The reaction is terminated by the addition of an acidic termination solution, and the absorbance is measured at 450 nm.

Background: 11β-HSD1 catalyzes the reversible conversion of cortisol to the inactive metabolite cortisone and plays a pathogenic role in obesity and metabolic syndrome. It could be utilized as a potential target for anti-obesity studies.

Synonyms: HSD11B1; Cortisone reductase; 11-beta-hydroxysteroid dehydrogenase type 1; NADPH-dependent 11β-HSD1; EC 1.1.1.146

Applications: Human 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) ELISA kit (30-9000 ng/L) is designed for the in vitro quantitative analysis of 11β-HSD1 levels in human serum, plasma, cell culture supernates, cell lysates, and tissue homogenate samples.

Research Area: Lipid metabolism; Hormone research; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernates; Cell lysates; Tissue homogenates

Detection Range: 30-9000 ng/L

Sensitivity: 15.19 ng/L

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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